The origin of ore-forming fluids and its relationship to deposit genesis are critical and difficult issues in study of mineral deposits. Taking the Haoyao erhudong gold deposit as an example, this work analyzes the microthermomtric data, in-situ laser Raman spectroscopy and chemical composi- tion of fluid inclusions and describes the ore-forming fluids systematically. The results show that the fluid inclusions in quartz can be divided into four cate- gories: two-phase inclusions of vapor and liquid, gaseous inclusions, pure gaseous inclusions, and pure liquid inclusions. Most fluid inclusions are ho- mogenized into liquids, while few inclusions are homogenized to gas phase. The homogenization temperature of the fluid inclusions in ore-bearing quartz veins is 260℃ - 380℃, and 260℃ - 340℃ for carbonaceous slates. Raman spectra of the inclusions indicate that water is the dominant component with amount of CH4 , N2 , H2 S and CO2 in the gaseous phase and C1 - , SO~- , Na + , K + , Mg2 + and Ca2 + in the liquid phase of the inclusimls. Hydrogen and oxygen isotope compositions (δDH2O = -96.20‰ - -82.80‰ δ^18 OH2O = 3.97‰- 7.93‰) suggest a mixing event between magmatic water and formation water. It is inferred that the gold mineralization may relate with the Hereynian magmatic hydrothermal activity. This gold deposit is of the meso- hypothermal type gold, which formed under low pressure in the shallow crust.