In this paper, the total amount, valence conversion and speciation of Fe in different deposit age waste from a large scale anaerobic landfill in southern Beijing were analyzed. The result shows that 1) the total amount of Fe was 18 532.8~26 897.2 mg/kg in 3 to 10-year waste; 2) Fe(III) is reduced to Fe(II) continuously and Fe(II)/Fe(III) ratio rises from 0.51 to 1.01 by dissimilatory iron reduction; and 3) the form distribution of Fe in different deposit age waste is generally identical: the highest content is residual fraction, following the reducible and oxidable fraction, and the acid soluble fraction is of the least. Reducible fraction is the most easily affected by environmental conditions, and sulfide has the most notable impact on the form distribution of Fe.