The Luoli gold deposit in Liping County of southeastern Guizhou Province is a low-sulfide gold-bearing quartz deposit. The characteristics of geology, quartz fluid inclusions homogenization temperature, salinity, and Raman spectrum suggest that the deposit formation process can be divided into 4 stages: vein formation stage, mineral formation stage, carbonate stage and supergene stage. The fluid inclusion composition is dominated by H2O, CO2, CH4, and N2. When the mineralization fluid of Au rose to the shallow subsurface along fissures, it caused metasomatic alteration with surrounding rocks, separating the fluids rich in H2O and CO2. In a certain sense, the content of CO2 can be used as an indicator for the exploration of gold deposits of quartz-vein type.