Pu-Wei Region is located in the northern part of central uplift zone, Dongpu Depression. Through utilizing the biomarker and nitrogen compounds to research the hydrocarbon migration characteristics in the region, it is found that there are two hydrocarbon injection areas. Hydrocarbon injection area is Wei42 block in the western flank of Pucheng-Weicheng sub-depression. At South Pucheng-Hubuzhai Region, the hydrocarbon comes from the high-mature hydrocarbon of Es3 reservoir in the southern Qianliyuan or Liutun sub-depressions except for the southern sub-depression of Pucheng-Weicheng Region. The vertical hydrocarbon migration shows the characteristics of vertical migration, which is mainly the chromatography.