Well SK1 is the first whole coring hole of the Cretaceous strata in China for scientific research. Different layers of shale in the Well SK1 (scientific well) in the SongliaoBasin were systematically analyzed by geochemical technique to determine the profile of total organic carbon content (TOC), maturity and depositional environment. The results show that the most organic-rich source rock is the Qingshankou 1st (K2qn1) formation with an average of TOC at 3.21% while most greater than 2%. Qingshankou 2nd~3rd (K2qn2+3) formations, Yaojia member (K2y) and Nenjiang 1st2nd (K2n1+2) formations have subordinate content of organic carbon. The vertical distribution of TOC shows nine HTB (high TOC at the base, decreasing upward) units, indicating the cyclicity of climate, biomass productivity and organic preservation. The source rock of Qingshankou 2nd~3rd formations became mature in the mid-depth with peak oil-generation at 1 500 m.