Annual soil dissolution rates of carbonate under forestland, grassland and shrub land at different depth in the Shuifang Spring in Jinfoshan, Chongqing are tested by means of the standard limestone tablets. The results show that the karst erosion rates are quite different under different land-use types, in different seasons and at different depth of soil. Finally, an estimation on the carbon sequestration in the spring area is done by means of standard limestone tablets, the result is about 25.595 t/a.