Thirty-seven crude oil samples and forty rock samples were analyzed by geochemistry analysis methods of GC-MS, carbon isotopic and light weight hydrocarbons for the further exploration research of Zhu Ⅲ depression. Based on the results, comprehensive comparison between oil source and hydrocarbon source rocks was done again and new cognition was gained. The results showed that the deep lacustrine facies source rock of Wenchang Formation is the major source rock of Wenchang B sag in Zhu Ⅲ depression, rather than the shore-shallow lacustrine facies source rock; and the swamp facies source rock of Enping Formation is the major source rock of Wenchang A sag in Zhu Ⅲ depression.