Mayinerbuo ophiolite located in Ertix-Mayinerbuo tectonic zone, layered gabbro and pillow basalt were dated using SHRIMP U-Pb method. The results indicate that concordant ages with a mean 206Pb/238U age of (397±6 ) Ma and (403±5) Ma, indicates that the Mayinerbuo Ophilit was supposed as relics of ocean basin in the northern Tianshan Mountains (Middle Palaeo-Asian oceanic crust) of the Late Silurian-Early Devonnian. The post orogenic pluton suggested that the collision of Siberia Plate and Kazakhstan-Jungger Plate before Early Permian.