Niutougang archaeological site is one of the largest ancient cultural sites in Nanjing. Phytolith analysis was carried out on its culture layers in order to reveal the climate circumstance that the ancestors experienced. Three stages can be identified according to phytolith assemblages. During late Neolithic period (before 3 500 cal. a B.P.), it was humid and warm. For the Shang Dynasty (3 500~3 000 cal. a B.P.), it was warmer and wetter than the previous stage. While during early West Zhou Dynasty(after 3 000 cal. a B.P.), there was a minor drop in both temperature and humidity, and the condition was analogous to that of first stage. This climatic history is consistent with global climate variations in mid-late Holocene, and is compatible with the profile suggested by cultural and mammal relics at the nearby Heyin Site. The optimum climate and environment during the late Neolithic to West Zhou Dynasty has favored the survival and reproduction of the ancestors, resulted in abundant cultural relics in Nanjing and the adjacent Chuhe region of this stage.