The Active Conformation of Allophycocyanin from Spirulina platensis Studied with Spectroscopic Analysis

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• 出版年：2010
• 作者：SHAO Si-mi；SU Hai-nan；ZHANG Xi-ying；PENG Guo-hong；ZHOU Bai-cheng；ZHANG Yu-zhong
• 单位1：State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University
• 出生年：1983
• 语种：中文
• 作者关键词：Allophyoocyanin；Absorption spectra；Fluorescence；Circular dichroism；Active confonnation
• 起始页：1643
• 总页数：4
• 经费资助：The work was supported by the National Natural Science Foundation of China (40806056)；the Hi-Tech Research and Development program of China (2008AA09Z404)and the Foundation for Outstanding Young Scientist (Doctoral Foundation) in Shandong Prov- ince(2008BS02019)
• 刊名：光谱学与光谱分析
• 是否内版：否
• 刊频：月刊
• 创刊时间：1981
• 主管单位：中国科学技术协会
• 主办单位：中国光学学会
• 主编：黄本立
• 地址：光谱学与光谱分析期刊社
• 邮编：100081
• 电子信箱：chngpxygpfx@vip.sina.com
• 网址：http://www.gpxygpfx.com
• 卷：30
• 期：6
• 期刊索取号：P34206220
• 数据库收录：中国物理类、化学类核心期刊；中国科协精品科技期刊；已被国内外CSCD，SCI，Ei，CA，AA，PЖ,MEDLINE等文献机构收录；中国科技论文统计源期刊；中国学术期刊文献统计源期刊
• 核心期刊：物理类、化学类核心期刊

摘要

Allophycocyanin (APC) was purified from Spirulina platensis using hydroxylapatite chromatogra-phy and ion-exchange chromatography. Effects of solution pH on spectra of APC were studied APC has anabsorption maximum at 650 nm, and a shoulder at 620 nm The fluorescence emission peak is at 660nm. Theefficiency of energy absorbing and transfer in APC could be reflected by the absorption spectra and fluorescencespectra, respectively. Structural variations of APC could be monitored by means of circular dichroism spectraAPC showed good absorbance and fluorescence stability at varying pH with only minor changes between pH 4-10. The trimeric structure of APC was maintained while local variations of protein peptides were allowed in re-sponse to the environmental disturbance Beyond this pH range, secondary structure as well as overaU confor-mation of APC dramatically changed, and the energy absorption and transfer ability were also disrupted