Water-Soluble Triarylborane Chromophores for One- and Two-Photon Excited Fluorescence Imaging of Mitochondria in Cells

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• 作者：Stefanie Griesbeck ; Dr. Zuolun Zhang ; Marcus Gutmann ; Dr. Tessa Lü ; hmann ; Dr. Robert M. Edkins ; Guillaume Clermont ; Dr. Adina N. Lazar ; Dr. Martin Haehnel ; Dr. Katharina Edkins ; Antonius Eichhorn ; Dr. Mireille Blanchard-Desce ; Prof. Dr. Lorenz Meinel and Prof. Dr. Todd B. Marder
• 刊名：Chemistry - A European Journal
• 出版年：2016
• 出版时间：October 4, 2016
• 年：2016
• 卷：22
• 期：41
• 页码：14701-14706
• 全文大小：2026K
• ISSN：1521-3765

文摘

Three water-soluble tetracationic quadrupolar chromophores comprising two three-coordinate boron π-acceptor groups bridged by thiophene-containing moieties were synthesised for biological imaging applications. Compound 3 containing the bulkier 5-(3,5-Me₂C₆H₂)-2,2′-(C₄H₂S)₂-5′-(3,5-Me₂C₆H₂) bridge is stable over a long period of time, exhibits a high fluorescence quantum yield and strong one- and two-photon absorption (TPA), and has a TPA cross section of 268 GM at 800 nm in water. Confocal laser scanning fluorescence microscopy studies in live cells indicated localisation of the chromophore at the mitochondria; moreover, cytotoxicity measurements proved biocompatibility. Thus, chromophore 3 has excellent potential for one- and two-photon-excited fluorescence imaging of mitochondrial function in cells.