Enzymatic Biodegradability of Pristine and Functionalized Transition Metal Dichalcogenide MoS₂ Nanosheets

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• 作者：Rajendra Kurapati ; Laura Muzi ; Aritz Perez Ruiz de Garibay ; Julie Russier ; Damien Voiry ; Isabella A. Vacchi ; Manish Chhowalla and Alberto Bianco
• 刊名：Advanced Functional Materials
• 出版年：2017
• 出版时间：February 17, 2017
• 年：2017
• 卷：27
• 期：7
• 全文大小：3292K
• ISSN：1616-3028

文摘

2D transition metal dichalcogenide MoS₂ nanosheets are increasingly attracting interests due to their promising applications in materials science and biomedicine. However, their biocompatibility and their biodegradability have not been thoroughly studied yet. Here, the biodegradability of exfoliated pristine and covalently functionalized MoS₂ (f-MoS₂) is investigated. First, biodegradability of these nanomaterials is evaluated using plant horseradish peroxidase and human myeloperoxidase. The results reveal that the enzymatic degradability rate of MoS₂ and f-MoS₂ is slower than in the case of the simple treatment with H₂O₂ alone. In parallel, high biocompatibility of both pristine and f-MoS₂ nanosheets is found up to 100 µg mL⁻¹ in both cell lines (HeLa and Raw264.7) and primary immune cells. In addition, no immune cell activation and minimal pro-inflammatory cytokine release are observed in RAW264.7 and human monocyte-derived macrophages, suggesting a negligible cellular impact of such materials. Furthermore, the effects of degraded MoS₂ and partially degraded f-MoS₂ products on cell viability and activation are studied in cancer and immune cells. A certain cytotoxicity is measured at the highest concentrations. Finally, to prove that the cellular impact is due to cell uptake, the internalization of both pristine and functionalized MoS₂ in cancer and primary immune cells is assessed.