A sensitive and selective LC-MS/MS method for the quantitative determination of segetalin A from the plasma of rats

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• 作者：Jianmeng Wang ; Nan Zhang ; Li Pang ; Dahai Xu ; Weiwei Yan ; Jiang Wu and Haiyan Chen
• 关键词：Vaccaria segetalis ; cyclic hexapeptide ; segetalin A ; pharmacokinetic study
• 刊名：Biomedical Chromatography
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：30
• 期：4
• 页码：606-611
• 全文大小：1032K
• ISSN：1099-0801

文摘

A sensitive and selective LC-MS/MS method was developed and validated for the determination and pharmacokinetic investigation of segetalin A in rat plasma. Sample preparation was accomplished through a simple SPE procedure for the removal and preconcentration of the analyte and IS. Plasma samples were separated by HPLC on a Symmetry C₁₈ column using a mobile phase consisting of methanol and 0.1% formic acid in water (70:30, v/v) with isocratic elution. The quantification was performed using multiple reaction monitoring with the transitions m/z 610.3 511.2 for segetalin A and m/z 779.4 751.4 for IS, respectively. The calibration curve was linear over the range of 8.0–4000 ng/mL with a limit of quantitation (LOQ) of 8.0 ng/mL. This method was applied in a pharmacokinetic study of segetalin A in rats. For intravenous (i.v.) administration, the plasma concentrations of segetalin A decreased quickly (t_1/2z, 1.31 ± 0.341 h). For oral administration, the plasma concentrations of segetalin A increased to a peak value at 1.50 ± 0.577 h, followed by a gradual decrease to the LOQ in 12 h. The mean AUC values after i.v. and oral administration were 553 ± 105 and 1482 ± 110 ng h/mL, respectively. Copyright