High genetic and virulence diversity detected in Neofusicoccum luteum and N. australe populations in New Zealand vineyards

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• 作者：J. Baskarathevan ; E. E. Jones ; M. V. Jaspers and H. J. Ridgway
• 刊名：Plant Pathology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：66
• 期：2
• 页码：268-276
• 全文大小：217K
• ISSN：1365-3059

文摘

Genotypic and virulence diversity of Neofusicoccum luteum and N. australe isolates recovered from grapevines displaying symptoms of dieback and decline in New Zealand were investigated. The universally primed PCR (UP-PCR) method was used to investigate the genetic diversity of 40 isolates of N. luteum and 33 isolates of N. australe. Five UP-PCR primers produced a total of 51 loci from N. luteum and 57 from N. australe with a greater number of polymorphic loci produced in N. australe (86%) compared with N. luteum (69%). Analysis of UP-PCR data showed both species found in New Zealand vineyards were genetically diverse at both the inter- and intra-vineyard levels with only a single pair of clonal isolates in N. luteum. Cluster analysis of UP-PCR data produced four genetic groups in N. luteum and 10 in N. australe (P < 0.05). For both species, there was no relationship between the genetic groups and the origin of isolates. The mean genetic diversity (H) of N. luteum was less than for N. australe, being 0.1791 and 0.2417, respectively. Pathogenicity assays of both species using isolates from either the same or different genetic groups inoculated onto either green shoots or grapevine trunks, showed virulence diversity within the population; however, no correlation was identified between genetic groups and virulence.