Repeated batch fermentation for surfactin production with immobilized Bacillus subtilis BS-37: two-stage pH control and foam fractionation

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• 作者：Gaobin Yi ; Qiang Liu ; Junzhang Lin ; Weidong Wang ; He Huang and Shuang Li
• 刊名：Journal of Chemical Technology and Biotechnology
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：92
• 期：3
• 页码：530-535
• 全文大小：623K
• ISSN：1097-4660

文摘

Surfactin, a lipopeptide biosurfactant produced by Bacillus strains, is one of the most surface-active biosurfactants, and has the potential to enhance oil recovery or environmental remediation. However, low yield and high production costs limit its use in high-volume applications. In this work, Bacillus subtilis BS-37 was immobilized on a piece of cotton towel in a 5 L stirred tank reactor; the fill-and-draw process and foam fractionation were used for repeated batch fermentations. A two-stage pH control operation was developed for surfactin fermentation: for repeated batch fermentation, the pH value was 5.0 for the biomass accumulation in the previous 6 h; and then the pH value was raised to 7.5 for surfactin production over the following 18 h of fermentation.