Biomimicry Promotes the Efficiency of a 10-Step Sequential Enzymatic Reaction on Nanoparticles, Converting Glucose to Lactate
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- 作者：Dr. Chinatsu Mukai ; Dr. Lizeng Gao ; Jacquelyn L. Nelson ; Dr. James P. Lata ; Dr. Roy Cohen ; Lauren Wu ; Meleana M. Hinchman ; Dr. Magnus Bergkvist ; Robert W. Sherwood ; Dr. Sheng Zhang and Dr. Alexander J. Travis
- 刊名：Angewandte Chemie
- 出版年：2017
- 出版时间：January 2, 2017
- 年：2017
- 卷：129
- 期：1
- 页码：241-244
- 全文大小：1883K
- ISSN：1521-3757
文摘
For nanobiotechnology to achieve its potential, complex organic–inorganic systems must grow to utilize the sequential functions of multiple biological components. Critical challenges exist: immobilizing enzymes can block substrate-binding sites or prohibit conformational changes, substrate composition can interfere with activity, and multistep reactions risk diffusion of intermediates. As a result, the most complex tethered reaction reported involves only 3 enzymes. Inspired by the oriented immobilization of glycolytic enzymes on the fibrous sheath of mammalian sperm, here we show a complex reaction of 10 enzymes tethered to nanoparticles. Although individual enzyme efficiency was higher in solution, the efficacy of the 10-step pathway measured by conversion of glucose to lactate was significantly higher when tethered. To our knowledge, this is the most complex organic–inorganic system described, and it shows that tethered, multi-step biological pathways can be reconstituted in hybrid systems to carry out functions such as energy production or delivery of molecular cargo.