Butanol production by a Clostridium beijerinckii mutant with high ferulic acid tolerance

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• 作者：Jun Liu ; Ting Guo ; Dong Wang ; Jiahui Xu and Hanjie Ying
• 刊名：Biotechnology and Applied Biochemistry
• 出版年：2016
• 出版时间：September/October 2016
• 年：2016
• 卷：63
• 期：5
• 页码：727-733
• 全文大小：381K
• ISSN：1470-8744

文摘

A mutant strain of Clostridium beijerinckii, with high tolerance to ferulic acid, was generated using atmospheric pressure glow discharge and high-throughput screening of C. beijerinckii NCIMB 8052. The mutant strain M11 produced 7.24 g/L of butanol when grown in P2 medium containing 30 g/L of glucose and 0.5 g/L of ferulic acid, which is comparable to the production from non-ferulic acid cultures (8.11 g/L of butanol). When 0.8 g/L of ferulic acid was introduced into the P2 medium, C. beijerinckii M11 grew well and produced 4.91 g/L of butanol. Both cell growth and butanol production of C. beijerinckii M11 were seriously inhibited when 0.9 g/L of ferulic acid was added into the P2 medium. Furthermore, C. beijerinckii M11 could produce 6.13 g/L of butanol using non-detoxified hemicellulosic hydrolysate from diluted sulfuric acid-treated corn fiber (SAHHC) as the carbon source. These results demonstrate that C. beijerinckii M11 has a high ferulic acid tolerance and is able to use non-detoxified SAHHC for butanol production.