Porous poly (D,L-lactide-co-glycolide) acid/biosilicate^® composite scaffolds for bone tissue engineering

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• 作者：Hueliton W. Kido ; Patricia Brassolatti ; Carla R. Tim ; Paulo R. Gabbai-Armelin ; Angela M.P. Magri ; Kelly R. Fernandes ; Paulo S. Bossini ; Nivaldo A. Parizotto ; Murilo C. Crovace ; Iran Malavazi ; Anderson F. da Cunha ; Ana M.G. Plepis ; Fernanda F. Anibal and Ana C.M. Rennó
• 刊名：Journal of Biomedical Materials Research Part B: Applied Biomaterials
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：105
• 期：1
• 页码：63-71
• 全文大小：540K
• ISSN：1552-4981

文摘

This study evaluated the effects of the Biosilicate^® and poly (D,L-lactic-co-glycolic) acid composites on bone repair in a tibial bone defect model in rats by means of using histological evaluation (histopathological and morphometric analysis) and gene expression analysis. Eighty male Wistar rats (12 weeks old, weighing ±300 g) were randomly divided into two groups: Biosilicate^® group (BG) and Biosilicate^®/PLGA group (BG/PLGA). Each group was euthanized at 3, 7, 14, and 21 days after surgery (n = 10 animals per time point). The main findings showed that the incorporation of PLGA into BG had a significant effect on the morphological structure of the material, accelerating mass loss, decreasing the pH and increasing the calcium release. Furthermore, histologic analysis revealed that the BG/PLGA showed increased material degradation, accompanied by higher bone formation compared to BG, after 21 days of implantation. In addition, qRT-PCR analysis showed that BG/PLGA induced an upregulation of the osteogenic genes related to BMP4, Runx2, ALP, and OC. These results show that the present BG/PLGA composite may be used as a bone graft for inducing bone repair.