Conformational Analysis of a High-Mannose-Type Oligosaccharide Displaying Glucosyl Determinant Recognised by Molecular Chaperones Using NMR-Validated Molecular Dynamics Simulation

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• 作者：Dr. Tatsuya Suzuki ; Megumi Kajino ; Dr. Saeko Yanaka ; Dr. Tong Zhu ; Dr. Hirokazu Yagi ; Dr. Tadashi Satoh ; Dr. Takumi Yamaguchi and Prof. Koichi Kato
• 刊名：ChemBioChem
• 出版年：2017
• 出版时间：February 16, 2017
• 年：2017
• 卷：18
• 期：4
• 页码：396-401
• 全文大小：1076K
• ISSN：1439-7633

文摘

Exploration of the conformational spaces of flexible oligosaccharides is essential to gain deeper insights into their functional mechanisms. Here we characterised dynamic conformation of a high-mannose-type dodecasaccharide with a terminal glucose residue, a critical determinant recognised by molecular chaperones. The dodecasaccharide was prepared by our developed chemoenzymatic technique, which uses ¹³C labelling and lanthanide tagging to detect conformation-dependent paramagnetic effects by NMR spectroscopy. The NMR-validated molecular dynamics simulation produced the dynamic conformational ensemble of the dodecasaccharide. This determined its spatial distribution as well as the glycosidic linkage conformation of the terminal glucose determinant. Moreover, comparison of our results with previously reported crystallographic data indicates that the chaperone binding to its target oligosaccharides involves an induced-fit mechanism.