Application of Z-sinapinic matrix in peptide MALDI-MS analysis

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• 作者：M. L. Salum ; S. L. Giudicessi ; T. Schmidt De Leó ; n ; S. A. Camperi and R. Erra-Balsells
• 刊名：Journal of Mass Spectrometry
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：52
• 期：3
• 页码：182-186
• 全文大小：426K
• ISSN：1096-9888c

文摘

Since introduction of sinapinic acid (SA) and α-cyano-4-hydroxycinnamic acid as matrices, successful application of matrix-assisted laser desorption/ionization mass spectrometry started for protein/polypeptides. Both show some limitations in short peptide analysis because matrix clusters are quite abundant. Cinnamics currently used are E-cinnamics. Here, Z-SA as matrix for peptides is studied and compared with E-SA and α-cyano-4-hydroxycinnamic acid. Minor number of clusters is always observed in the low m/z region allowing the detection of short peptides. The results here described show that this novel matrix is a tool of choice for direct, rapid and sensitive detection of hydrophilic and hydrophobic peptides. Copyright