Magnetic mesoporous enzyme-silica composites with high activity and enhanced stability
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- 作者：Jiandong Cui ; Yuxiao Feng ; Shu Yue ; Yamin Zhao ; Linbo Li ; Ronglin Liu and Tao Lin
- 关键词：enzyme immobilization ; biomimetic silica ; magnetic enzyme&ndash ; silica composites ; phenylalanine ammonia lyase ; mesoporous materials
- 刊名：Journal of Chemical Technology & Biotechnology
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：91
- 期：6
- 页码：1905-1913
- 全文大小：1315K
- ISSN：1097-4660
文摘
Encapsulation of enzymes in biomimetic silica seems to be a promising immobilization method due to mild processing conditions and short preparation time. However, biomimetic silica particles may be considered too small for industrial applications because they result in difficult recovery. Furthermore, the use of R5 peptide or polymers as catalyst usually causes protein denaturation. To overcome these drawbacks, a new stable and recyclable biocatalyst based on co-encapsulation of magnetic nanoparticles and phenylalanine ammonia lyase (PAL) in biomimetic silica has been prepared using enzyme-assisted direct condensation reactions of silicon oxide with tetramethoxysilane (TMOS).