Highly Efficient Intramolecular Electrochemiluminescence Energy Transfer for Ultrasensitive Bioanalysis of Aflatoxin M1
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- 作者：Jia-Li Liu ; Dr. Min Zhao ; Prof. Ying Zhuo ; Prof. Ya-Qin Chai and Prof. Ruo Yuan
- 刊名：Chemistry - A European Journal
- 出版年：2017
- 出版时间：February 3, 2017
- 年：2017
- 卷：23
- 期：8
- 页码：1853-1859
- 全文大小：1123K
- ISSN：1521-3765
文摘
The intermolecular electrochemiluminescence resonance energy transfer (ECL-RET) between luminol and Ru(bpy)32+ was studied extensively to achieve the sensitive bioanalysis owing to the perfect spectral overlap of the donor and acceptor, but it still suffers from the challenging issue of low energy-transfer efficiency. The intramolecular ECL-RET towards the novel ECL compound containing the donor of luminol and the acceptor of Ru(bpy)2(mcpbpy)2+ (Lum-Ru) was designed and investigated. With the high-efficient ECL-RET in one molecule, the highly intense ECL signal of Lum-Ru was obtained owing to the short path of energy transmission and less energy loss between luminol and Ru(bpy)2(mcpbpy)2+. Lum-Ru was further applied to construct a signal-off electrochemiluminescence (ECL) aptasensor for ultrasensitive detection of a harsh carcinogen of Aflatoxin M1 (AFM1). This sensing platform also provides a significant boost for the trace detection of other biomolecules in clinical analysis.