Cellular internalization and morphological analysis after intravenous injection of a highly hydrophilic octahedral rhenium cluster complex - a new promising X-ray contrast agent

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• 作者：Anna A. Krasilnikova ; Anastasiya O. Solovieva ; Kristina E. Trifonova ; Konstantin A. Brylev ; Anton A. Ivanov ; Sung-Jin Kim ; Michael A. Shestopalov ; Maria S. Fufaeva ; Alexander M. Shestopalov ; Yuri V. Mironov ; Alexander F. Poveshchenko and Lidia V. Shestopalova
• 刊名：Contrast Media & Molecular Imaging
• 出版年：2016
• 出版时间：November/December 2016
• 年：2016
• 卷：11
• 期：6
• 页码：459-466
• 全文大小：700K
• ISSN：1555-4317

文摘

The octahedral cluster compound Na₂H₈[{Re₆Se₈}(P(C₂H₄CONH₂)(C₂H₄COO)₂)₆] has been shown to be highly radio dense, thus becoming a promising X-ray contrast agent. It was also shown that this compound had low cytotoxic effect in vitro, low acute toxicity in vivo and was eliminated rapidly from the body through the urinary tract. The present contribution describes a more detailed cellular internalization assay and morphological analysis after intravenous injection of this hexarhenium cluster compound at different doses. The median lethal dose (LD₅₀) of intravenously administrated compound was calculated (4.67 ± 0.69 g/kg). Results of the study clearly indicated that the cluster complex H_n[{Re₆Se₈}(P(C₂H₄CONH₂)(C₂H₄COO)₂)₆]^n–10 was not internalized into cells in vitro and induced only moderate morphological alterations of kidneys at high doses without any changes in morphology of liver, spleen, duodenum, or heart of mice. Copyright