Design, synthesis, and negative inotropic evaluation of 4-phenyl-1H-1,2,4-triazol-5(4H)-one derivatives containing triazole or piperazine moieties

详细信息    查看全文

• 作者：Zhi-Yu Wei ; Bai-Ri Cui ; Xun Cui ; Yan-Ling Wu ; Yang Fu ; Li-Ping Liu and Hu-Ri Piao
• 刊名：Chemical Biology & Drug Design
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：89
• 期：1
• 页码：47-60
• 全文大小：1020K
• ISSN：1747-0285

文摘

In this study, four novel series of 4-phenyl-1H-1,2,4-triazol-5(4H)-one derivatives containing triazole or piperazine moieties were designed, synthesized, and evaluated for negative inotropic activity by measuring the left atrium stroke volume in isolated rabbit heart preparations. Almost all of the compounds showed an ability to moderate the cardiac workload by decreasing the heart rate and contractility. Among them, 7h was found to be the most potent with a change in stroke volume of −48.22 ± 0.36% at a concentration of 3 × 10⁻⁵ mol/L (metoprolol: −9.74 ± 0.14%). The cytotoxicity of these compounds was evaluated using the human cervical cancer cell line HeLa, the liver cancer cell line Hep3B, and the human normal hepatic cell line LO2. A preliminary study of the mechanism of action for the compound 7h on the regulation of atrial dynamics with ATP-sensitive K⁺ channel and L-type Ca²⁺ channel blockers glibenclamide and nifedipine was performed in the isolated perfused beating rabbit atria.