High Performance, Multiplexed Lung Cancer Biomarker Detection on a Plasmonic Gold Chip

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• 作者：Bin Liu ; Yaling Li ; Hao Wan ; Lin Wang ; Wei Xu ; Shoujun Zhu ; Yongye Liang ; Bo Zhang ; Jiatao Lou ; Hongjie Dai and Kun Qian
• 刊名：Advanced Functional Materials
• 出版年：2016
• 出版时间：November 22, 2016
• 年：2016
• 卷：26
• 期：44
• 页码：7994-8002
• 全文大小：1171K
• ISSN：1616-3028

文摘

Diagnosis of lung cancer is performed using a plasmonic gold (pGOLD) chip through multiplexed near-infrared (NIR) detection of carcino-embryonic antigen (CEA), Cyfra21-1, and neuron-specific enolase (NSE) in the serum samples of patients. With ≈50-fold enhancement of NIR fluorescence, multiplexed microarray analysis of CEA, Cyfra21-1, and NSE in 10 μL of human serum or whole blood samples on pGOLD chip leads to markedly improved limit-of-quantification, limit-of-detection, reproducibility, and higher diagnostic sensitivity and specificity compared to traditional biochips and Luminex technology currently in use in hospitals.