MicroRNA profile analysis of host cells before and after wild human rotavirus infection

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• 作者：Yan Zhou ; Jinyuan Wu ; Panpan Geng ; Xiang Kui ; Yuping Xie ; Lei Zhang ; Yaling Liu ; Na Yin ; Guangming Zhang ; Shan Yi ; Hongjun Li and Maosheng Sun
• 刊名：Journal of Medical Virology
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：88
• 期：9
• 页码：1497-1510
• 全文大小：1876K
• ISSN：1096-9071

文摘

Rotavirus infection is an important cause of acute gastroenteritis in children, but the interaction between rotavirus and host cells is not completely understood. We isolated a wildtype (wt) rotavirus strain, ZTR-68(P [8] G1), which is derived from an infant with diarrhea in southwest China in 2010. In this study, we investigated host cellular miRNA expression profiles changes in response to ZTR-68 in early stage of infection to investigate the role of miRNAs upon rotavirus infection. Differentially expressed miRNAs were identified by deep sequencing and qRT-PCR and the function of their targets predicted by Gene Ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway annotation. A total of 36 candidate miRNAs were identified. Comparative analysis indicated that 29 miRNAs were significantly down-regulated and 7 were up-regulated after infection. The data were provided contrasting the types of microRNAs in two different permissive cell lines (HT29 and MA104). The target assays results showed that mml-miR-7 and mml-miR-125a are involved in anti-rotavirus and virus–host interaction in host cells. These results offer clues for identifying potential candidates in vector-based antiviral strategies. J. Med. Virol. 88:1497–1510, 2016.