A Single Gene Cluster for Chalcomycins and Aldgamycins: Genetic Basis for Bifurcation of Their Biosynthesis
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- 作者：Xiao-Long Tang ; Ping Dai ; Prof. Dr. Hao Gao ; Dr. Chuan-Xi Wang ; Dr. Guo-Dong Chen ; Prof. Dr. Kui Hong ; Dr. Dan Hu and Prof. Dr. Xin-Sheng Yao
- 刊名：ChemBioChem
- 出版年：2016
- 出版时间：July 1, 2016
- 年：2016
- 卷：17
- 期：13
- 页码：1241-1249
- 全文大小：842K
- ISSN：1439-7633
文摘
Aldgamycins are 16-membered macrolide antibiotics with a rare branched-chain sugar d-aldgarose or decarboxylated d-aldgarose at C-5. In our efforts to clone the gene cluster for aldgamycins from a marine-derived Streptomyces sp. HK-2006-1 capable of producing both aldgamycins and chalcomycins, we found that both are biosynthesized from a single gene cluster. Whole-genome sequencing combined with gene disruption established the entire gene cluster of aldgamycins: nine new genes are incorporated with the previously identified chalcomycin gene cluster. Functional analysis of these genes revealed that almDI/almDII, (encoding α/β subunits of pyruvate dehydrogenase) triggers the biosynthesis of aldgamycins, whereas almCI (encoding an oxidoreductase) initiates chalcomycins biosynthesis. This is the first report that aldgamycins and chalcomycins are derived from a single gene cluster and of the genetic basis for bifurcation in their biosynthesis.