Three-Layered PCL Grafts Promoted Vascular Regeneration in a Rabbit Carotid Artery Model
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- 作者：Kai Wang ; Wenting Zheng ; Yiwa Pan ; Shaoyang Ma ; Yong Guan ; Ruming Liu ; Meifeng Zhu ; Xin Zhou ; Jun Zhang ; Qiang Zhao ; Yan Zhu ; Lianyong Wang and Deling Kong
- 关键词：cellular infiltration ; electrospinning ; poly(&epsilon ; -caprolactone) ; tissue engineering ; vascular grafts
- 刊名：Macromolecular Bioscience
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：16
- 期：4
- 页码：608-618
- 全文大小：4066K
- ISSN：1616-5195
文摘
In this study, a three layered poly (ε-caprolactone) (PCL) graft (tPCL) was fabricated by electrospinning PCL and electrospraying poly (ethylene oxide) (PEO), which has a thin dense inner layer, a loose middle layer, and a dense outer layer. Regular PCL grafts (rPCL) with only a dense layer were used as control. In vivo evaluation was performed in rabbit carotid artery. Enhanced cell infiltration, rapid regeneration of endothelium and smooth muscle layers, and increased elastin deposition were observed within the tPCL graft wall. After 3 months, tPCL grafts showed faster PCL degradation than the rPCL grafts. Infiltrated macrophages in the tPCL grafts secreted higher level of monocyte chemoattractant protein-1 (MCP-1) and vascular endothelial growth factor (VEGF) which enhanced vascular regeneration. In conclusion, the tPCL graft may be a useful vascular prosthesis and worth for further investigation.