Real-Time Imaging of Cell Behaviors in Living Organisms by a Mitochondria-Targeting AIE Fluorogen

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• 作者：Bo Situ ; Sijie Chen ; Engui Zhao ; Chris Wai Tung Leung ; Yilong Chen ; Yuning Hong ; Jacky Wing Yip Lam ; Zilong Wen ; Wei Liu ; Wenqing Zhang ; Lei Zheng and Ben Zhong Tang
• 刊名：Advanced Functional Materials
• 出版年：2016
• 出版时间：October 18, 2016
• 年：2016
• 卷：26
• 期：39
• 页码：7132-7138
• 全文大小：969K
• ISSN：1616-3028

文摘

Visualizing behaviors of cell populations within living multicellular organisms in real time is of great value to life science but challenging due to the lack of ideal probes. In this work, a biocompatible fluorogen, azide-functionalized tetraphenylethene pyridinium (TPE-PyN₃), is reported for noninvasive imaging and sensing within living systems. TPE-PyN₃ exhibits unique aggregation-induced emission (AIE) attributes and high affinity to mitochondria, enabling it to achieve specific mitochondrial imaging and long-term cellular observing with excellent photostability both in vitro and in vivo. The high membrane penetrability of TPE-PyN₃ allows all of the cells within the living zebrafish embryos to be morphologically visualized and reconstructed in 3D. Moreover, TPE-PyN₃ is capable of indicating cell apoptosis because of its sensitivity to the change of mitochondrial membrane potential. The findings presented here provide a simple and noninvasive tool for studying behaviors of cell populations in vivo for the first time by a small-molecule AIE probe.