Modified filter-aided sample preparation (FASP) method increases peptide and protein identifications for shotgun proteomics

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• 作者：Mao-wei Ni ; Lu Wang ; Wei Chen ; Han-zhou Mou ; Jie Zhou and Zhi-guo Zheng
• 刊名：Rapid Communications in Mass Spectrometry
• 出版年：2017
• 出版时间：30 January 2017
• 年：2017
• 卷：31
• 期：2
• 页码：171-178
• 全文大小：506K
• ISSN：1097-0231

文摘

Mass spectrometry (MS)-based protein identification depends mainly on protein extraction and digestion. Although sodium dodecyl sulfate (SDS) can preclude enzymatic digestion and interfere with MS analysis, it is still the most widely used surfactant in these steps. To overcome these disadvantages, a SDS-compatible proteomic technique for SDS removal prior to MS-based analyses was developed, namely filter-aided sample preparation (FASP).