Skin aging caused by intrinsic or extrinsic processes characterized with functional proteomics

详细信息    查看全文

• 作者：Jia-You Fang ; Pei-Wen Wang ; Chun-Hsun Huang ; Mu-Hong Chen ; Yun-Ru Wu and Tai-Long Pan
• 刊名：PROTEOMICS
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：16
• 期：20
• 页码：2718-2731
• 全文大小：5122K
• ISSN：1615-9861

文摘

The skin provides protection against environmental stress. However, intrinsic and extrinsic aging causes significant alteration to skin structure and components, which subsequently impairs molecular characteristics and biochemical processes. Here, we have conducted an immunohistological investigation and established the proteome profiles on nude mice skin to verify the specific responses during aging caused by different factors. Our results showed that UVB-elicited aging results in upregulation of proliferating cell nuclear antigen and strong oxidative damage in DNA, whereas chronological aging abolished epidermal cell growth and increased the expression of caspase-14, as well as protein carbonylation. Network analysis indicated that the programmed skin aging activated the ubiquitin system and triggered obvious downregulation of 14-3-3 sigma, which might accelerate the loss of cell growth capacity. On the other hand, UVB stimulation enhanced inflammation and the risk of skin carcinogenesis. Collectively, functional proteomics could provide large-scale investigation of the potent proteins and molecules that play important roles in skin subjected to both intrinsic and extrinsic aging.