Analysis of mandipropamid residual levels through systematic method optimization against the matrix complexity of sesame leaves using HPLC/UVD

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• 作者：Waziha Farha ; Md. Musfiqur Rahman ; A. M. Abd El-Aty ; Sung-Woo Kim ; Da-I Jung ; So Jeong Im ; Jeong-Heui Choi ; Md. Humayun Kabir ; Kang-Bong Lee ; Ho-Chul Shin and Jae-Han Shim
• 刊名：Biomedical Chromatography
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：30
• 期：7
• 页码：990-995
• 全文大小：381K
• ISSN：1099-0801

文摘

An analytical method was developed to detect mandipropamid residues in sesame leaves using high-performance liquid chromatography–ultraviolet detection. Samples were extracted with acetonitrile and were prepurified using a solid-phase extraction (SPE) cartridge with an additional dispersive-SPE (d-SPE) sorbent application. The method was validated using an external calibration curve prepared using pure solvent. The linearity was excellent with determination coefficient = 1. The limits of detection and quantification were 0.003 and 0.01 mg/kg, respectively. Recoveries at three spiking levels – 0.1, 0.5, and 1.0 mg/kg – were in the range 80.3–90.7% with relative standard deviations <2%. This method was applied to field-treated samples collected from two different areas, Gwangju and Muan, in the Republic of Korea and the half-lives were similar, 5.10 and 5.41 days, respectively. The pre-harvest residue limit was also predicted for both sites. The proposed method is sensitive and able to quantify trace amounts of mandipropamid in leafy vegetables. The combination of SPE and d-SPE effectively removed the matrix components in sesame leaves. Copyright