OleD Loki as a Catalyst for Tertiary Amine and Hydroxamate Glycosylation
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- 作者：Ryan R. Hughes ; Dr. Khaled A. Shaaban ; Dr. Jianjun Zhang ; Dr. Hongnan Cao ; Prof. George N. Phillips Jr. and Prof. Jon S. Thorson
- 刊名：ChemBioChem
- 出版年：2017
- 出版时间：February 16, 2017
- 年：2017
- 卷：18
- 期：4
- 页码：363-367
- 全文大小：1197K
- ISSN：1439-7633
文摘
We describe the ability of an engineered glycosyltransferase (OleD Loki) to catalyze the N-glycosylation of tertiary-amine-containing drugs and trichostatin hydroxamate glycosyl ester formation. As such, this study highlights the first bacterial model catalyst for tertiary-amine N-glycosylation and further expands the substrate scope and synthetic potential of engineered OleDs. In addition, this work could open the door to the discovery of similar capabilities among other permissive bacterial glycosyltransferases.