Development of the 19 X-STR loci multiplex system and genetic analysis of a Zhejiang Han population in China

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• 作者：XingYi Yang ; WeiWei Wu ; LinLi Chen ; ChangHui Liu ; XiaoFang Zhang ; Ling Chen ; XingLin Feng ; HuiJun Wang and Chao Liu
• 刊名：ELECTROPHORESIS
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：37
• 期：15-16
• 页码：2260-2272
• 全文大小：1884K
• ISSN：1522-2683

文摘

The 19 X-STRs multiplex system is a PCR-based amplification kit that facilitates simultaneous amplification of 19 X-chromosomal STR loci (i.e. DXS7423, DXS10148, DXS10159, DXS6809, DXS7424, DXS8378, DXS10164, DXS10162, DXS7132, DXS10079, DXS6789, DXS101, DXS10103,DXS10101, HPTRB, DXS10075, DXS10074, DXS10135, and DXS10134). Eleven loci were extensively used in an Investigator Qiagen Argus X-12 (DXS7423, DXS10148, DXS8378, DXS10162, DXS7132, DXS10079, DXS10103, DXS10101, HPTRB, DXS10074, and DXS10135). In this research, the multiplex system was tested for detection sensitivity, DNA mixtures, inhibitor tolerance and species specificity; SWGDAM Validation Guidelines – Approved December 2012 were followed for the human fluorescent STR multiplex PCR reagent. Samples from 181 unrelated Zhejiang Han individuals (121 males and 60 females) were typed using this multiplex system. The results show that this 19X-STRs multiplex system is a robust and reliable amplification means to facilitate forensic and human identification testing.