Ultrasensitive electrochemical immunoassay for carcinoembryonic antigen based on three-dimensional macroporous gold nanoparticles/graphene composite platform and multienzyme functionalized nanoporous silver label

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• 作者：Guoqiang Sun^a ; Juanjuan Lu^a ; Shenguang Ge^b ; Xianrang Song^c ; Jinghua Yu^a ; ^{ujn.yujh@gmail.com} ; ^{306375767@qq.com} ; Mei Yan^a ; Jiadong Huang^a
• 关键词：Three-dimensional gold nanoparticles/graphene composite ; Nanoporous silver ; Carcinoembryonic antigen ; Electrochemical immunosensor
• 刊名：Analytica Chimica Acta
• 出版年：2013
• 出版时间：2 May, 2013
• 年：2013
• 卷：775
• 期：Complete
• 页码：85-92
• 全文大小：1973 K

文摘

Three-dimensional macroporous gold nanoparticles/graphene composites (3D-AuNPs/GN) were synthesized through a simple two-step process, and were used to modify working electrode sensing platform, based on which a facile electrochemical immunoassay for sensitive detection of carcinoembryonic antigen (CEA) in human serum was developed. In the proposed 3D-AuNPs/GN, AuNPs were distributed not just on the surface, but also on the inside of graphene. And this distribution property increased the area of sensing surface, resulting in capturing more primary antibodies as well as improving the electronic transmission rate. In the presence of CEA, a sandwich-type immune composite was formed on the sensing platform, and the horseradish peroxidase-labeled anti-CEA antibody (HRP-Ab₂)/thionine/nanoporous silver (HRP-Ab₂/TH/NPS) signal label was captured. Under optimal conditions, the electrochemical immunosensor exhibited excellent analytical performance: the detection range of CEA is from 0.001 to 10 ng mL^?1 with low detection limit of 0.35 pg mL^?1 and low limit of quantitation (LOQ) of 0.85 pg mL^?1. The electrochemical immunosensor showed good precision, acceptable stability and reproducibility, and could be used for the detection of CEA in real samples. The proposed method provides a promising platform of clinical immunoassay for other biomolecules