Antioxidant and in vivo genoprotective effects of phenolic compounds identified from an endophytic Cladosporium velox and their relationship with its host plant Tinospora cordifolia

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• 作者：Bahaderjeet Singh^a ; ^{sidhubahaderjit@yahoo.in} ; Prince Sharma^b ; ^{princeranayal@gmail.com} ; Arun Kumar^c ; ^{arunbiochemju@gmail.com} ; Pooja Chadha^b ; ^{poojachadha77@yahoo.co.in} ; Ramandeep Kaur^c ; ^{gndu.ramandeep@gmail.com} ; Amarjeet Kaur^a ; ^{amarjeet_b@rediffmail.com}
• 关键词：Gallic acid (pubchem CID ; 370) ; catechin (pubchem CID ; 9064) ; chlorogenic acid (pubchem CID ; 1794427) ; epicatechin (pubchem CID ; 72276) ; caffeic acid (pubchem CID ; 689043) ; umbelliferon (pubchem CID ; 5281426) ; coumaric acid (pubchem CID ; 637542) ; ellagic acid (pubchem CID ; 5281855)
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：24 December 2016
• 年：2016
• 卷：194
• 期：Complete
• 页码：450-456
• 全文大小：639 K
• 卷排序：194

文摘

Tinospora cordifolia (Willd. Hook. f. & Thomson; family: Menispermaceae), has a long history of use in various traditional medicinal systems including “Ayurveda”. It is reported to possess anticancer, antidiabetic, antimicrobial, antispasmodic, and antiinflammatory activities. T. cordifolia has also been well documented for production of various bioactive metabolites and their antioxidant activity, but the microorganisms associated with it have been least explored for the same properties.Aim of the studyAim of the present study was to evaluate antioxidant and in vivo genoprotective potential of phenolic compounds produced by an endophytic fungus Cladosporium velox TN-9S isolated from T. cordifolia.Materials and methodsThe isolate of C. velox TN-9S was cultivated in malt extract medium and extracted with ethyl acetate. Total phenol content was determined by Folin Ciocalteu reagent. The antioxidant activity was measured in terms of DPPH and FRAP assay. The in vivo genoprotective activity was assessed using fish Channa punctatus as model. Identification of phenolic compounds was carried out using RP-HPLC. The fungal extract was evaluated for biosafety using Salmonella typhimurium His- strain and CHO cell lines for mutagenicity and cytotoxicity, respectively.ResultsThe total phenolic content in the ethyl acetate extract of the fungus was determined to be 730 μg gallic acid equivalent/mL. The extract evinced significant antioxidant activity with IC50 value of 22.5 µg/mL in DPPH scavenging assay. The phenolic extract showed good in vivo genoprotective activity against the genetic damage induced in fish C. punctatus after treatment with a non-ionic surfactant 4-nonylphenol. RP-HPLC analysis revealed the presence of peaks corresponding to various phenolic compounds in the extract. Mutagenicity and cytotoxicity results revealed the extract to be nonmutagenic and non cytotoxic in nature.ConclusionThe results indicate the potential of an endophytic C. velox isolated from T. cordifolia as a producer of phenolic compounds with antioxidant and genoprotective activities which could be exploited in pharmaceutical industry. The ability of endophytes to produce similar compounds as the host, is also revealed in the present study.