Decreased cruzipain and gp85/trans-sialidase family protein expression contributes to loss of Trypanosoma cruzi trypomastigote virulence

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• 作者：Juan San Francisco^a ; Iv&aacute ; n Barrí ; a^b ; Bessy Gutié ; rrez^a ; Iv&aacute ; n Neira^a ; Christian Muñ ; oz^a ; Hern&aacute ; n Sagua^a ; Jorge E. Araya^a ; Juan Carlos Andrade^a ; Anibal Zailberger^c ; Alejandro Catal&aacute ; n^a ; Francisco Remonsellez^d ; José ; Luis Vega^b ; Jorge Gonz&aacute ; lez^a ; ^{jorge.gonzalez@uantof.cl}
• 关键词：Trypanosoma cruzi ; Virulence ; Genetically related cell lines ; Cruzipain ; Gp85/trans-sialidase family
• 刊名：Microbes and Infection
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：19
• 期：1
• 页码：55-61
• 全文大小：582 K
• 卷排序：19

文摘

Two cell lines derived from a single Trypanosoma cruzi clone by long-term passaging generated a highly virulent (C8C3hvir) and a low virulent (C8C3lvir) cell line. The C8C3hvir cell line was highly infective and lethal to Balb/c mice, and the C8C3lvir cell line was three- to five-fold less infective to mouse cardiomyocytes than C8C3hvir. The highly virulent T. cruzi cell line abundantly expressed the major cysteine proteinase cruzipain (Czp), complement regulatory protein (CRP) and trans-sialidase (TS), all of which are known to act as virulence factors in this parasite. The in vitro invasion capacity and in vivo Balb/c mouse infectiveness of the highly virulent strain was strongly reduced by pre-treatment with antisense oligonucleotides targeting TS or CRP or with E64d. Based on these results, we conclude that decreased levels of TS, CRP and Czp expression could contribute to loss of T. cruzi trypomastigote virulence.