Temperature-dependent acetoin production by Pyrococcus furiosus is catalyzed by a biosynthetic acetolactate synthase and its deletion improves ethanol production
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- 作者：Diep M.N. Nguyena ; Gina L. Lipscomba ; Gerrit J. Schuta ; Brian J. Vaccaroa ; Mirko Basena ; 1 ; Robert M. Kellyb ; Michael W.W. Adamsa ; adams@bmb.uga.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：ALS ; α-acetolactate synthase ; ALDC ; α-acetolactate decarboxylase ; AOR ; aldehyde ferredoxin oxidoreductase ; AHAS ; α-acetohydroxy acid synthase ; ILV ; isoleucine ; leucine and valine ; POR ; pyruvate ferredoxin oxidoreductase ; ACSI ; acetyl-CoA synthase I ; DM ; defined medium
- 刊名：Metabolic Engineering
- 出版年：2016
- 出版时间：March 2016
- 年：2016
- 卷：34
- 期：Complete
- 页码：71-79
- 全文大小：1370 K
文摘
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The heterotrophic archaeon Pyrococcus furiosus (Pf) grows optimally at 100 °C.
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Pf was previously engineered to produce ethanol near 75 °C.
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Pf shown to generate acetoin (3-hydroxybutanone) as a major end product below 80 °C.
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Biosynthetic acetolactate synthase (ALS) is the enzyme that produces acetoin.
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A als deletion mutant has increased ethanol production.