Determination of the distributions of degrees of acetylation of chitosan

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• 作者：Joel Jerushan Thevarajah^a ; ^b ; ^{joel.thevarajah@westernsydney.edu.au} ; Matthew Paul Van Leeuwen^a ; ^c ; ^{m.vanleeuwen@westernsydney.edu.au} ; Herve Cottet^d ; ^{herve.cottet@umontpellier.fr} ; Patrice Castignolles^b ; ^{p.castignolles@westernsydney.edu.au} ; Marianne Gaborieau^a ; ^b ; ^{m.gaborieau@westernsydney.edu.au}
• 关键词：Chitosan ; Distribution of degrees of acetylation ; Capillary electrophoresis
• 刊名：International Journal of Biological Macromolecules
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：95
• 期：Complete
• 页码：40-48
• 全文大小：2124 K
• 卷排序：95

文摘

Chitosan is often characterized by its average degree of acetylation. To increase chitosan’s use in various industries, a more thorough characterization is necessary as the acetylation of chitosan affects properties such as dissolution and mechanical properties of chitosan films. Despite the poor solubility of chitosan, free solution capillary electrophoresis (CE) allows a robust separation of chitosan by the degree of acetylation. The distribution of degrees of acetylation of various chitosan samples was characterized through their distributions of electrophoretic mobilities. These distributions can be obtained easily and with high precision. The heterogeneity of the chitosan chains in terms of acetylation was characterized through the dispersity of the electrophoretic mobility distributions obtained. The relationship between the number-average degree of acetylation obtained by solid-state NMR spectroscopy and the weight-average electrophoretic mobilities was established. The distribution of degrees of acetylation was determined using capillary electrophoresis in the critical conditions (CE-CC).