Design and synthesis of affinity chromatography ligands for the purification of 5-hydroxyeicosanoid dehydrogenase

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• 作者：Chintam Nagendra Reddy^a ; ^&dagger ; ; Qiuji Ye^a ; ^&dagger ; ; Pranav Patel^a ; Sashikala Sivendran^a ; Shishir Chourey^a ; Rui Wang^a ; Jaganmohan R. Anumolu^a ; Gail E. Grant^b ; ^&Dagger ; ; William S. Powell^b ; Joshua Rokach^a ; ^{jrokach@fit.edu}
• 关键词：AA ; arachidonic acid ; 5-HETE ; 5-hydroxy-6E ; 8Z ; 11Z ; 14Z-eicosatetraenoic acid ; 5-oxo-ETE ; 5-oxo-6E ; 8Z ; 11Z ; 14Z-eicosatetraenoic acid ; 5-HEDH ; 5-hydroxyeicosanoid dehydrogenase ; 5-HpETE ; 5-hydroperoxyeicosatetraenoic acid ; LT ; leukotriene ; NADP+ ; nicotinamide adenine dinucleotide phosphate ; TMS ; tetramethylsilane ; HPLC ; high-performance liquid chromatography
• 刊名：Bioorganic & Medicinal Chemistry
• 出版年：2017
• 出版时间：1 January 2017
• 年：2017
• 卷：25
• 期：1
• 页码：116-125
• 全文大小：1258 K
• 卷排序：25

文摘

Arachidonic acid (AA) is converted to biologically active metabolites by different pathways, one of the most important of which is initiated by 5-lipoxygenase (5-LO). 5-Hydroxyeicosatetraenoic acid (5-HETE), although possessing only weak biological activity itself, is oxidized to 5-oxo-6,8,11,14-eicosatetraenoic acid (5-oxo-ETE), a potent chemoattractant for eosinophils and neutrophils. Our main goal is to determine how the biosynthesis of 5-oxo-ETE is regulated and to determine its pathophysiological roles. To achieve this task, we designed and synthesized affinity chromatography ligands for the purification of 5-hydroxyeicosanoid dehydrogenase (5-HEDH), the enzyme responsible for the formation of 5-oxo-ETE.