Immobilized biomass in AnIBPR removes COD at low temperature and short HRT. COD removal by AnIBPR was achieved at 15–35 °C. Dissolved CH4 were oversaturated at saturation ratio of 1.02–1.21. Low KLa limited liquid-to-gas mass transfer of dissolved CH4 at cold temperature. AnIBPR performance was not impeded at low VFA contents.