Development of data-independent acquisition workflows for metabolomic analysis on a quadrupole-orbitrap platform

详细信息    查看全文

• 作者：Juntuo Zhou^a ; Yuhua Li^a ; Xi Chen^a ; Lijun Zhong^b ; ^{zhonglijun@bjmu.edu.cn} ; Yuxin Yin^a ; ^c ; ^d ; ^{yinyuxin@bjmu.edu.cn}
• 关键词：Liquid chromatograph ; Mass spectrometer ; Data-dependent acquisition ; Metabolomics ; Orbitrap ; Papillary thyroid carcinoma
• 刊名：Talanta
• 出版年：2017
• 出版时间：1 March 2017
• 年：2017
• 卷：164
• 期：Complete
• 页码：128-136
• 全文大小：1143 K
• 卷排序：164

文摘

DIA workflow is constructed on Q-Orbitrap platform for metabolomics analysis. HILIC/RPLC-DIA-MS workflows are constructed for analysis of metabolites and lipids. Performances of DDA, DIA, and AIF strategies were compared and discussed. Serum of the thyroid carcinoma patients is analyzed by DIA-MS to reveal perturbed metabolites.