Concerted action of activation-induced cytidine deaminase and uracil-DNA glycosylase reduces covalently closed circular DNA of duck hepatitis B virus
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- 作者：Sajeda Chowdhury ; Kouichi Kitamura ; Miyuki Simadu ; Miki Koura ; Masamichi Muramatsu
- 关键词：AID ; activation-induced cytidine deaminase ; APOBEC ; apolipoprotein B mRNA-editing enzyme catalytic subunit ; UNG ; uracil-DNA glycosylase ; DHBV ; duck hepatitis B virus ; cccDNA ; covalently closed circular DNA ; 3D-PCR ; differential denaturation DNA PCR
- 刊名：FEBS Letters
- 出版年：2013
- 出版时间：17 September, 2013
- 年：2013
- 卷：587
- 期：18
- 页码：3148-3152
- 全文大小：967 K
文摘
Covalently closed circular DNA (cccDNA) forms a template for the replication of hepatitis B virus (HBV) and duck HBV (DHBV). Recent studies suggest that activation-induced cytidine deaminase (AID) functions in innate immunity, although its molecular mechanism of action remains unclear, particularly regarding HBV restriction. Here we demonstrated that overexpression of chicken AID caused hypermutation and reduction of DHBV cccDNA levels. Inhibition of uracil-DNA glycosylase (UNG) by UNG inhibitor protein (UGI) abolished AID-induced cccDNA reduction, suggesting that the AID/UNG pathway triggers the degradation of cccDNA via cytosine deamination and uracil excision.