Preparation of Mouse Embryonic Stem Cells and Cardiomyocyte Differentiation Induced with Retinoic Acid and Ascorbic Acid

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• 作者：Zhao Xun-wu^* ; ^{806161051@qq.com" class="auth_mail" title="E-mail the corresponding author} ; Zheng Peng ; Huang Zhi-jun ; Zeng Yue ; E O Adegoke ; Zhang Gui-xue ; ^{gxzhang@neau.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：embryonic stem cell ; differentiation ; retinoic acid ; ascorbic acid
• 刊名：Journal of Northeast Agricultural University (English Edition)
• 出版年：2015
• 出版时间：September 2015
• 年：2015
• 卷：22
• 期：3
• 页码：62-66
• 全文大小：834 K

文摘

The experiment was designed to study effects of retinoic acid and ascorbic acid on differentiation of mouse embryonic stem cells to cardiomyocytes. Embryonic bodies (EB) were developed from mESC in suspension culture, different levels of concentration of retinoic acid and ascorbic acid were used to determine the optimal conditions for EB formation. The results showed that the optimal concentrations were 10⁻⁹ mol • L⁻¹ and 0.1 mg • mL⁻¹ for retinoic acid and ascorbic acids, respectively. 50% of EB which was significantly (p<0.05) different from the control group developed to cardiomyocytes. In conclusion, retinoic acid and ascorbic acid had strong ability to promote cardiomyocyte differentiation of mouse embryonic stem cells. 10⁻⁹ mol • L⁻¹ retinoic acid and 0.10 mg • mL⁻¹ ascorbic acids were recommended to induce differentiation of mouse ES cells toward cardiomyocytes.