A comparison in therapeutic efficacy of several time points of intravenous StemBell administration in a rat model of acute myocardial infarction
详细信息 查看全文
- 作者：Reindert W. Emmens1 ; 2 ; 3 ; * ; r.emmens@vumc.nl ; Maikel Oedayrajsingh-Varma1 ; 2 ; * ; Linde Woudstra1 ; 2 ; Otto Kamp2 ; 4 ; 5 ; Elisa Meinster1 ; Annemieke van Dijk1 ; Marco N. Helder6 ; Diana Wouters3 ; Sacha Zeerleder3 ; 7 ; S. Marieke van Ham3 ; Nico de Jong5 ; 8 ; Hans W.M. Niessen1 ; 2 ; 9 ; Lynda J.M. Juffermans2 ; 4 ; 5 ; Paul A.J. Krijnen1 ; 2
- 关键词：adipose tissue ; intravenous administration ; microbubbles ; myocardial infarction ; stromal cells
- 刊名：Cytotherapy
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：19
- 期：1
- 页码：131-140
- 全文大小：1269 K
- 卷排序：19
文摘
Adipose-derived stromal cells (ASCs) are a promising new therapeutic option for patients with acute myocardial infarction (AMI). Previously, we found that ASCs coupled to antibody-targeted microbubbles (StemBells [StBs]) improved cardiac function when administered intravenously 7 days post-AMI in rats. In this study, we compared the efficacy of intravenous StB administration at different administration time points following AMI in rats.MethodsAMI, followed by reperfusion, was induced in four groups of male Wistar rats, which subsequently received an intravenous 1 × 106 StB bolus 1 day post-AMI (StB1; n = 8), 7 days post-AMI (StB7; n = 9), at both time points (StB1+7; n = 7) or neither (Control; n = 7). The effect onrdiac function was determined using echocardiography prior to AMI, 7 days post-AMI and 42 days post-AMI. The effect on infarct size and macrophages in the infarct core were determined (immuno)histochemically 42 days post-AMI.ResultsAt 42 days post-AMI, all three StB groups had a significantly improved fractional shortening compared with the control group. Between the StB-treated groups, the effects did not differ significantly at 42 days post-AMI. At 7 days post-AMI, the StB1 group had a significantly improved fractional shortening compared with the control and StB7 groups. No significant changes in infarct size or macrophage numbers were found compared with the control group for any StB group.ConclusionsStB administration resulted in long-term improvement of cardiac function, independent of the time point of administration. When administered at 1 day post-AMI, this improvement was already evident at 7 days post-AMI.