- 作者：Saadia Naseema ; saadia.naseem@comsats.edu.pk" class="auth_mail" title="E-mail the corresponding author ; Stephan Winterb
- 关键词：African cassava mosaic virus ; East African cassava mosaic virus ; Single and mix infections ; Genome components ; Kinetic PCR
- 刊名：Journal of Virological Methods
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：227
- 期：Complete
- 页码：23-32
- 全文大小：2110 K
- •
The quantity of genomic DNA-A and DNA-B of African cassava mosaic virus and East African cassava mosaic virus Uganda was analysed by quantitative PCR to assess virus concentrations in susceptible and tolerant cassava cultivars in infections.
- •
The concentrations of genome components in absolute and relative quantification experiments in single and mixed viral infections were determined.
- •
The quantitative assessment showed that the distribution of both viruses in the moderately resistant cassava cv. TMS 30572 was not different from the highly susceptible cv. TME 117.
- •
Natural mixed infections with both viruses presented severe disease symptoms and differential amounts of genome components.
- •
The higher concentrations of EACMV-UG DNA-B compared to EACMV DNA-A accumulation in single infections were consistent and irrespective of cassava cultivar and its tolerance status.