Pharmacological effect of methanol extracts of excellent- and low-grade radixes was compared using assay of inhibition of acetylcholine-induced contraction in guinea pig ileum. Pharmacological contents of the radix were quantitatively measured by HPLC analysis.
Excellent-grade radixes showed lower activity than low-grade radixes on the inhibition assay for ileum contraction. (Z)-Ligustilide content of the excellent-grade radixes was statistically significantly lower than that of other grades of radixes (p-value by Student's t-test: 6.9 × 10−4). A regression equation was obtained from assessing the inhibitory activity of a (Z)-ligustilide standard at various concentrations. Comparison of the inhibitory activity values, which were estimated by the regression equation, and the actual activity values, which were determined using the assay, indicated that ligustilide is the factor that dominantly affects the activity. The ligustilide concentration of hot water extracts of radixes was determined. The concentration in the hot water extracts and the content of the radixes was positively correlated based on a Pearson correlation test. This result suggests that ligustilide extracted by infusion depends on its radix content.
There are differences in (Z)-ligustilide content and its pharmacological activity depending on the grade of Japanese Angelica radix.