Self-assembling of poly(aspartic acid) with bovine serum albumin in aqueous solutions

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• 作者：L.E. Nita^a ; ^{lnazarie@yahoo.co.uk} ; A.P. Chiriac^a ; M. Bercea^a ; M. Asandulesa^a ; Bernhard A. Wolf^b
• 关键词：Interpolymer complex ; Bovine serum albumin ; Poly(aspartic acid) ; Self-assembling
• 刊名：International Journal of Biological Macromolecules
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：95
• 期：Complete
• 页码：412-420
• 全文大小：1705 K
• 卷排序：95

文摘

Macromolecular co-assemblies built up in aqueous solutions, by using a linear polypeptide, poly(aspartic acid) (PAS), and a globular protein, bovine serum albumin (BSA), have been studied. The main interest was to identify the optimum conditions for an interpenetrated complex formation in order to design materials suitable for biomedical applications, such as drug delivery systems. BSA surface possesses several amino- and carboxylic groups available for covalent modification, and/or bioactive substances attachment. In the present study, mixtures between PAS and BSA were investigated at 37 °C in dilute aqueous solution by viscometry, dynamic light scattering and zeta potential determination, as well as in solid state by AFM microscopy and dielectric spectroscopy. The experimental data have shown that the interpolymer complex formation occurs for a PAS/BSA molar ratio around 0.541.