Combining micro-computed tomography with histology to analyze biomedical implants for peripheral nerve repair
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- 作者：Tracy M. Hopkinsa ; Alexander M. Heilmana ; James A. Liggetta ; Kathleen LaSanceb ; Kevin J. Littlec ; David B. Homd ; Danielle M. Minteere ; Kacey G. Marraf ; g ; Sarah K. Pixleya ; Sarah.pixley@uc.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：DAPI ; 4&prime ; 6-diamidino-2-phenylindole ; anti-NF ; antibody to neurofilament protein 200 ; DICOM file format ; digital imaging and communications in medicine file format ; DTI ; diffusion tensor imaging ; GLUT-1 ; the glucose transporter-1 protein ; H&E ; hematoxylin and eosin ; I&minus ; iodide ions ; IKI or I2KI ; iodine potassium iodide ; LM ; light microscopy ; micro-CT ; micro-computed tomography ; I2 ; molecular iodine ; MRI ; molecular resonance imaging ; PBS ; phosphate buffered saline ; PET ; positron emissi
- 刊名：Journal of Neuroscience Methods
- 出版年：2015
- 出版时间：30 November 2015
- 年：2015
- 卷：255
- 期：Complete
- 页码：122-130
- 全文大小：2568 K
文摘
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Imaged ex vivo iodinated normal and repaired nerves with micro-CT.
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Removed iodine with sodium thiosulfate, allowing subsequent histology.
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Removal of iodine did not alter immunostaining or cellular morphology.
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Matching CT to staining improved studies of nerve regeneration through conduits.
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Micro-CT with iodine allowed imaging of soft tissues adjacent to metal implants.