Altered mitochondrial dynamics and response to insulin in cybrid cells harboring a diabetes-susceptible mitochondrial DNA haplogroup
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- 作者：Hsiao-Mei Kuoa ; b ; 1 ; Shao-Wen Wenga ; 1 ; Alice Y.W. Changc ; Hung-Tu Huangd ; Hung-Yu Lina ; Jiin-Haur Chuange ; Tsu-Kung Linf ; Chia-Wei Liouf ; Ming-Hong Taig ; Ching-Yi Lina ; Pei-Wen Wanga ; 1 ; wangpw@adm.cgmh.org.tw" class="auth_mail" title="E-mail the corresponding author
- 关键词：Cybrid ; cytoplasmic hybrid ; DAPI ; 4&prime ; 6-diamidino-2-phenylindole ; DMEM ; Dulbecco&rsquo ; s Modified Eagle Medium ; DRP1 ; dynamin-related protein 1 ; ECAR ; extracellular acidification rate ; FCCP ; carbonylcyanide-p-trifluoromethoxyphenylhydrazone ; FIS1 ; mitochondrial fission 1 protein ; GLUT1/GLUT4 ; Glut1/Glut4 glucose transporters ; H2O2 ; hydrogen peroxide ; IR ; insulin resistance ; JNK ; c-Jun N-terminal kinase ; MFN1 and MFN2 ; mitofusins 1 and 2 ; mtDNA ; mitochondrial DNA ; NAC ; N-acetyl-L-cysteine ; O
- 刊名：Free Radical Biology and Medicine
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：96
- 期：Complete
- 页码：116-129
- 全文大小：5120 K
文摘
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Mitochondria have an independent role in the pathogenesis of insulin resistance.
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Cybrid B4 showed fragmented mitochondria, impaired biogenesis and bioenergetics.
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Insulin-induced increase in mitochondrial function and network were only in cybrid D4.
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The defects in cybrid B4 were improved in response to antioxidant treatment.